Description
pUC19 DNA is isolated from E.coli (dam+, dcm+) and purified by ultracentrifugation twice through a cesium chloride gradient in the presence of ethidium bromide. The molecule is a double-stranded circle, 2686 base pairs in length. GenBank/EMBL accession number L09137.
pUC19 is a plasmid vector suitable for cloning and for the dideoxy DNA sequencing method. It has multiple cloning sites in the lacZ' region. Since the multiple cloning site is inserted into the 5' end of the lac Z' gene, insertion of foreign DNA in this region will destroy the alpha complementation activity of the lac Z' gene product, making the screening for white colonies on plates containing X-Gal and IPTG possible.
Recombinant plasmids generate white colonies while non-recombinant plasmids generate blue colonies.
DNA cloned into pUC19 can be end-labeled while still in the plasmid and sequenced without further DNA purification.
pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of 1.8x10e6 daltons and contains 2686 base pairs.
CAP protein binding site - 591-554 (compl. strand); mRNA (LacZ) starts at nt position 507 (compl. strand); lac repressor binding site - 507-487 (compl. strand)